Prokaryotic cells are tiny but mighty, packing a punch without fancy organelles. They're simpler than eukaryotes, with DNA floating freely and smaller ribosomes. But don't underestimate them – these little guys are incredibly diverse and adaptable.
From spheres to rods to spirals, prokaryotes come in all shapes and sizes. They've got tough cell walls, handy plasmids, and some even sport flagella for swimming. Bacteria and archaea may look similar, but they've got some key differences under the hood.
Defining Characteristics and Morphology of Prokaryotic Cells
Key features of prokaryotic cells
- Lack membrane-enclosed organelles resulting in DNA located directly in the cytoplasm, not within a nucleus
- Possess smaller ribosomes (70S) compared to eukaryotic ribosomes (80S) which are sites of protein synthesis
- Contain a single, circular chromosome with a generally smaller genome size relative to eukaryotes (E. coli, Streptococcus)
- May possess extrachromosomal DNA in the form of plasmids which are small, circular DNA molecules that replicate independently
- Cell wall present external to the cell membrane provides structural support and protection
- Peptidoglycan key component in bacterial cell walls (Staphylococcus aureus)
- Archaeal cell walls lack peptidoglycan and have distinct structures (pseudomurein, S-layer)
- Smaller in size compared to eukaryotic cells, typically ranging from 0.1-5 μm in diameter (Mycoplasma, Escherichia coli)
Shapes and arrangements of prokaryotes
- Shapes
- Cocci: spherical (Streptococcus)
- Bacilli: rod-shaped (Escherichia coli)
- Spirilla: spiral or curved (Spirillum)
- Vibrios: comma-shaped (Vibrio cholerae)
- Arrangements
- Diplococci: pairs of cocci (Neisseria)
- Streptococci: chains of cocci (Streptococcus pyogenes)
- Staphylococci: grape-like clusters of cocci (Staphylococcus aureus)
- Tetrads: square arrangement of four cocci (Micrococcus)
- Sarcinae: cube-like arrangement of eight cocci (Sarcina)
- Streptobacilli: chains of bacilli (Streptobacillus moniliformis)
Structure and Function of Prokaryotic Cell Components
Structure and function of prokaryotic components
- Cell envelope
- Cell membrane: phospholipid bilayer serves as a selective barrier controlling passage of substances
- Cell wall: provides structural support, protection, and maintains cell shape
- Gram-positive bacteria have a thick peptidoglycan layer (Bacillus)
- Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane (Salmonella)
- Capsule: external polysaccharide layer provides additional protection and aids in adherence to surfaces (Streptococcus pneumoniae)
- Cytoplasm: interior of the cell where metabolic reactions and processes occur
- Ribosomes: sites of protein synthesis, smaller (70S) than eukaryotic ribosomes
- Inclusions: storage granules for nutrients (glycogen, polyphosphate) and other substances
- Nucleoid: region where the single, circular DNA chromosome is located, not enclosed by a nuclear membrane
- Plasmids: small, extrachromosomal DNA molecules that can replicate independently and often carry genes for antibiotic resistance
- Pili and fimbriae: surface appendages involved in attachment to surfaces and DNA transfer during conjugation (F pilus)
- Flagella: long, whip-like structures used for motility, composed of flagellin protein (Escherichia coli)
Bacterial vs archaeal cell characteristics
- Similarities
- Both lack membrane-bound organelles
- Possess a single, circular DNA chromosome located in the cytoplasm
- Ribosomes (70S) are present in the cytoplasm for protein synthesis
- Differences
- Cell wall composition
- Bacterial cell walls contain peptidoglycan (murein)
- Archaeal cell walls lack peptidoglycan and may have pseudomurein, S-layer, or other unique structures
- Membrane lipids
- Bacterial membranes have ester-linked lipids
- Archaeal membranes have ether-linked lipids which are more stable in extreme conditions
- RNA polymerase
- Bacteria have a single type of RNA polymerase
- Archaea have multiple types of RNA polymerase, similar to eukaryotes
- Metabolic diversity
- Archaea exhibit unique metabolic capabilities, such as methanogenesis (methanogens) and survival in extreme environments (hyperthermophiles, halophiles)
Prokaryotic Cell Processes
- Cell division: occurs through binary fission, where a single cell divides into two identical daughter cells
- Metabolism: diverse range of metabolic pathways for energy production and biosynthesis, including aerobic and anaerobic respiration, fermentation, and photosynthesis
- Motility: achieved through various mechanisms such as flagella, gliding, or twitching motility, allowing cells to move towards favorable environments or away from harmful ones