Stokes shift refers to the difference in energy between the absorbed photon and the emitted photon in a fluorescence process. This phenomenon occurs because when a molecule absorbs light, it transitions to an excited state, and as it returns to the ground state, it loses some energy through non-radiative processes, resulting in longer wavelengths of emitted light. The significance of Stokes shift lies in its application to techniques that utilize fluorescence, allowing for better signal detection and differentiation between the excitation and emission spectra.
congrats on reading the definition of Stokes shift. now let's actually learn it.