Stokes Shift refers to the phenomenon where the wavelength of light emitted by a fluorescent molecule is longer than the wavelength of light absorbed. This shift occurs due to the loss of energy as the molecule transitions from an excited state back to its ground state, often involving vibrational relaxation. The Stokes Shift is crucial in distinguishing the emitted fluorescence from the excitation light in techniques like UV-visible and fluorescence spectroscopy.
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