Steady-state fluorescence refers to the constant fluorescence intensity emitted by a sample when it is continuously excited by a light source at a fixed wavelength. This state occurs after the initial transient phase of fluorescence, where the rate of excitation matches the rate of emission, allowing for a stable measurement of fluorescence properties. Understanding steady-state fluorescence is crucial in analyzing quantum yield and fluorescence lifetime, as it provides insights into the efficiency and duration of excited state populations.
congrats on reading the definition of Steady-State Fluorescence. now let's actually learn it.