Nucleic acid amplification testing

Definition

Nucleic acid amplification testing (NAAT) is a molecular technique used to detect specific genetic material from pathogens. It is highly sensitive and can identify infections even when the pathogen is present in very low quantities.

5 Must Know Facts For Your Next Test

1. NAAT is commonly used to diagnose urogenital protozoan infections like Trichomoniasis.
2. This method includes techniques such as PCR (Polymerase Chain Reaction), which amplifies DNA sequences.
3. NAAT can provide results faster than traditional culture methods, often within a few hours.
4. It has a high specificity and sensitivity, reducing the likelihood of false positives and negatives.
5. Samples for NAAT can be obtained from urine, vaginal swabs, or urethral swabs.

Review Questions

• What are the advantages of using NAAT over traditional culture methods in diagnosing urogenital infections?
• Which types of samples are suitable for nucleic acid amplification testing?
• How does NAAT improve the detection of low-quantity pathogens?

Related terms

Polymerase Chain Reaction (PCR): A technique used in NAAT to amplify specific DNA sequences.

Trichomoniasis: A protozoan infection of the urogenital tract caused by Trichomonas vaginalis.

Sensitivity: The ability of a test to correctly identify those with the disease (true positive rate).