Microarray analysis

Definition

Microarray analysis is a technique used to study the expression of many genes simultaneously by measuring mRNA levels. It involves hybridizing cDNA to a grid of DNA probes on a solid surface.

5 Must Know Facts For Your Next Test

1. Microarray analysis can identify changes in gene expression under different conditions.
2. Fluorescent labeling is commonly used to detect hybridization signals in microarray analysis.
3. Data from microarray experiments require extensive bioinformatics for interpretation.
4. Microarrays can be used to compare gene expression profiles between different cell types or tissues.
5. They are instrumental in understanding complex genetic interactions and pathways.

Review Questions

• What is the main purpose of microarray analysis?
• How are hybridization signals detected in microarray analysis?
• Why is bioinformatics important in interpreting microarray data?

Related terms

cDNA: Complementary DNA synthesized from an RNA template by reverse transcription.

Hybridization: The process of forming a double-stranded nucleic acid from two complementary strands.

Bioinformatics: The application of computational tools to process and analyze biological data.