Confirmatory test

Definition

A confirmatory test is a secondary assay performed to validate the results obtained from an initial screening test, ensuring accuracy and specificity. It is essential in eliminating false positives and confirming the presence of specific antibodies or antigens.

5 Must Know Facts For Your Next Test

1. Confirmatory tests provide higher specificity compared to initial screening tests.
2. They are crucial in clinical diagnostics for verifying diseases like HIV, hepatitis, and other immune responses.
3. Common confirmatory tests include Western Blot, PCR (Polymerase Chain Reaction), and ELISA (Enzyme-Linked Immunosorbent Assay).
4. These tests often require more time, resources, and expertise than preliminary tests.
5. Confirmatory testing minimizes the risk of diagnostic errors, ensuring that treatment decisions are based on accurate data.

Review Questions

• What is the primary purpose of a confirmatory test?
• Name two common types of confirmatory tests used in immunology.
• Why are confirmatory tests important in clinical diagnostics?

Related terms

Screening Test: A preliminary test used to detect potential indicators of a disease or condition.

Western Blot: A laboratory method used to detect specific proteins within a sample through gel electrophoresis and antibody binding.

ELISA: An assay technique that uses enzymes linked to antibodies or antigens to detect and quantify substances such as peptides, proteins, antibodies, and hormones.