Phosphoenolpyruvate (PEP) is a high-energy compound that plays a crucial role in glycolysis, serving as a key substrate for the enzyme pyruvate kinase. It is formed from 2-phosphoglycerate through an enzymatic reaction catalyzed by enolase. PEP is notable for its energy content, which is used to transfer a phosphate group to ADP, ultimately generating ATP during the final steps of glycolysis.
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