Size exclusion chromatography (SEC) is a separation technique that allows for the separation of molecules based on their size as they pass through a column filled with porous material. In this method, smaller molecules can enter the pores of the stationary phase and take longer to travel through the column, while larger molecules are excluded from these pores and elute faster. This technique is particularly useful for purifying proteins, polysaccharides, and other macromolecules, as it helps to achieve high resolution and minimal sample loss.
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