Phosphositeplus

Definition

Phosphositeplus is a comprehensive database and resource that provides detailed information about phosphorylation sites on proteins, along with associated biological data. It connects phosphorylation events with various aspects such as protein function, structure, and interactions, making it a vital tool for understanding post-translational modifications (PTMs) in proteomics.

5 Must Know Facts For Your Next Test

1. Phosphositeplus integrates data from various experimental sources, including mass spectrometry and literature, providing a rich resource for researchers studying phosphorylation.
2. The database allows users to search for specific proteins or phosphorylation sites, making it easier to identify critical PTMs related to disease states or biological processes.
3. Phosphositeplus includes information about the kinases responsible for phosphorylating specific sites, linking enzymatic activity with phosphorylation events.
4. It also provides insights into the functional consequences of phosphorylation, such as changes in protein interactions or cellular localization.
5. The platform supports hypothesis generation by allowing researchers to visualize phosphorylation patterns across different conditions or in various biological contexts.

Review Questions

• How does phosphositeplus contribute to our understanding of protein phosphorylation and its biological implications?
  • Phosphositeplus serves as a valuable resource by providing detailed data on phosphorylation sites and their biological significance. By integrating information from multiple sources, it enables researchers to link specific phosphorylation events to functional outcomes in proteins. This understanding helps clarify how post-translational modifications impact processes like cell signaling and disease mechanisms.
• Discuss the significance of including kinase information within phosphositeplus in relation to phospho-regulation.
  • Including kinase information in phosphositeplus is crucial because kinases are the enzymes that add phosphate groups to proteins, thereby regulating their activity. By identifying which kinases target specific phosphorylation sites, researchers can better understand the signaling pathways involved and how dysregulation of these kinases might contribute to diseases. This insight into phospho-regulation is key for developing targeted therapies.
• Evaluate how the comprehensive data provided by phosphositeplus can influence research directions in proteomics and related fields.
  • The comprehensive data from phosphositeplus can significantly influence research directions by providing a foundational resource for hypothesis generation and experimental design. Researchers can utilize this data to identify key phosphorylation sites that may play roles in disease or cellular response mechanisms. Furthermore, by highlighting patterns across different conditions, it may inspire new studies focused on specific pathways or interactions affected by phosphorylation, ultimately advancing our understanding of proteomics and its applications.

Related terms

Phosphorylation: The process of adding a phosphate group to a protein or other organic molecule, which can alter its function and activity.

Post-translational Modifications (PTMs): Chemical modifications that occur to proteins after their synthesis, which can affect their stability, localization, and activity.

Proteomics:

The large-scale study of proteins, particularly their functions and structures, often involving techniques to analyze protein expression and modifications.