Microbiology

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Total magnification

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Microbiology

Definition

Total magnification is the product of the magnifying power of the ocular lens and the objective lens in a microscope. It determines how much larger an object appears compared to its actual size.

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5 Must Know Facts For Your Next Test

  1. Total magnification is calculated by multiplying the ocular lens magnification by the objective lens magnification.
  2. Common ocular lenses have a magnification of 10x, while objective lenses vary, typically ranging from 4x to 100x.
  3. High total magnification does not always mean better resolution; other factors like numerical aperture also play a role.
  4. In compound microscopes, achieving high total magnification requires proper alignment and focus of both lenses.
  5. Understanding total magnification is crucial for accurately interpreting microscopic images and measurements.

Review Questions

  • How is total magnification calculated in a microscope?
  • What are typical magnifications for ocular and objective lenses?
  • Why is high total magnification not always indicative of better image quality?

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