Sonication

Definition

Sonication is the use of high-frequency sound waves to disrupt cell structures. It is commonly used in microbiology for lysing cells and reducing microbial load.

5 Must Know Facts For Your Next Test

1. Sonication can effectively break down cell walls and membranes, leading to cell lysis.
2. It generates cavitation bubbles that collapse, producing intense shear forces.
3. Sonication is often used to homogenize samples or disperse particles in a solution.
4. The effectiveness of sonication depends on factors like power output, duration, and temperature.
5. Sonication can also be used in combination with other physical or chemical methods to enhance antimicrobial effects.

Review Questions

• What is the primary mechanism by which sonication disrupts microbial cells?
• How does the effectiveness of sonication vary with different parameters such as power output and duration?
• In what scenarios might sonication be combined with other antimicrobial methods?

Related terms

Autoclaving:

A sterilization method using saturated steam under pressure to kill all forms of microorganisms.

Filtration:

A physical method for separating microbes from solutions based on size exclusion.

UV Radiation:

Using ultraviolet light to damage DNA and inactivate microorganisms.