SDS-PAGE

Definition

SDS-PAGE is a technique used to separate proteins based on their molecular weight. It utilizes sodium dodecyl sulfate (SDS) to denature proteins and polyacrylamide gel electrophoresis (PAGE) to resolve them.

5 Must Know Facts For Your Next Test

1. SDS-PAGE separates proteins by their size, not their charge or shape.
2. Sodium dodecyl sulfate (SDS) is an anionic detergent that gives proteins a uniform negative charge.
3. The gel matrix in SDS-PAGE is made of polyacrylamide, which forms a mesh-like structure.
4. After separation, proteins can be visualized using staining techniques such as Coomassie Brilliant Blue or silver staining.
5. Molecular weight markers are often run alongside samples for determining the approximate size of separated proteins.

Review Questions

• What role does SDS play in SDS-PAGE?
• How does the polyacrylamide gel function in separating proteins?
• What are two common methods used to visualize proteins after SDS-PAGE?

Related terms

Electrophoresis: A method used to separate charged molecules under the influence of an electric field.

Western Blotting:

A technique that uses antibodies to detect specific proteins separated by SDS-PAGE.

Isoelectric Focusing:

A technique for separating proteins based on their isoelectric point.