Primary antibody
from class: Microbiology Definition A primary antibody is an immunoglobulin that binds specifically to a target antigen, enabling its detection and study. It is the first antibody used in immunoassays like ELISA to detect the presence of an antigen.
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Predict what's on your test 5 Must Know Facts For Your Next Test Primary antibodies are directly involved in binding to specific antigens. They are essential for the detection of specific proteins in techniques such as Western blotting, immunohistochemistry, and ELISA. The specificity of a primary antibody determines the accuracy of antigen detection. Primary antibodies can be monoclonal or polyclonal depending on their source and specificity. Proper selection and validation of primary antibodies are critical for successful experimental outcomes. Review Questions What is the role of a primary antibody in an ELISA? How does the specificity of a primary antibody affect experimental results? What are the differences between monoclonal and polyclonal primary antibodies?
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