Immunocytochemistry (ICC)

Definition

Immunocytochemistry (ICC) is a laboratory technique used to visualize the presence and localization of specific proteins or antigens in cells by using labeled antibodies as specific probes. It combines immunological and biochemical methods to identify cellular components.

5 Must Know Facts For Your Next Test

1. ICC uses antibodies that are conjugated with fluorescent dyes or enzymes to detect specific proteins within cells.
2. Samples for ICC are typically fixed and permeabilized cells, which allows antibodies to access intracellular targets.
3. The primary antibody binds specifically to the target antigen, while a secondary antibody, if used, binds to the primary antibody and is usually labeled for detection.
4. ICC can be quantitatively analyzed using techniques like fluorescence microscopy or flow cytometry.
5. This technique is crucial for studying cell biology, pathology, and immune responses at the cellular level.

Review Questions

• What is the role of the primary antibody in Immunocytochemistry?
• Why is cell fixation important in ICC?
• How can ICC results be detected and analyzed?

Related terms

Immunohistochemistry (IHC):

A method similar to ICC but applied to tissue sections instead of individual cells.

Flow Cytometry:

A technique used to detect and measure physical and chemical characteristics of a population of cells or particles.

Enzyme-Linked Immunosorbent Assay (ELISA):

A plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.