Microbiology

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Illuminator

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Microbiology

Definition

An illuminator is a component in a microscope that provides the light necessary to view specimens. It plays a crucial role in achieving optimal contrast and resolution.

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5 Must Know Facts For Your Next Test

  1. The illuminator can be an internal or external light source, such as an LED or halogen lamp.
  2. Proper alignment of the illuminator is essential for Köhler illumination, which ensures even lighting across the specimen.
  3. Adjustable intensity settings on the illuminator allow for better control over image brightness and contrast.
  4. The type of illuminator used can affect the color temperature and quality of the observed image.
  5. In fluorescence microscopy, specialized illuminators like mercury or xenon arc lamps are used to excite fluorophores.

Review Questions

  • What is the function of an illuminator in a microscope?
  • Why is proper alignment of the illuminator important for Köhler illumination?
  • How does changing the intensity setting on an illuminator affect the microscopic image?

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