Fluorogen
from class: Microbiology Definition A fluorogen is a molecule that becomes fluorescent upon binding with another specific molecule or undergoing a chemical reaction. Fluorogens are commonly used in biological assays to detect the presence of target substances.
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Predict what's on your test 5 Must Know Facts For Your Next Test Fluorogens are essential in immunoassays like EIAs and ELISAs for detecting specific antigens or antibodies. In fluorescent antibody techniques, fluorogens help visualize the binding of antibodies to their antigens under a fluorescence microscope. Fluorogens require activation by light of a specific wavelength to emit fluorescence. The intensity of the fluorescence emitted by fluorogens is proportional to the amount of target substance present in the sample. Commonly used fluorogens include fluorescein, rhodamine, and phycoerythrin. Review Questions What role do fluorogens play in ELISA tests? How does a fluorogen become fluorescent? Name three commonly used fluorogens in laboratory assays.
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