Fluorescent enzyme immunoassays (FEIAs)

Definition

Fluorescent enzyme immunoassays (FEIAs) are laboratory techniques used to detect the presence of antibodies or antigens in a sample by using fluorescent-labeled enzymes. The fluorescence emitted is measured to determine the concentration of the target molecule.

5 Must Know Facts For Your Next Test

1. FEIAs use fluorescent-labeled enzymes as markers to detect specific antibodies or antigens.
2. The intensity of fluorescence correlates with the concentration of the target molecule in the sample.
3. FEIAs can be used for both qualitative and quantitative analysis.
4. They provide high sensitivity and specificity compared to other immunoassays.
5. Common applications include diagnosing infectious diseases, detecting allergens, and monitoring therapeutic drug levels.

Review Questions

• What is the role of fluorescent-labeled enzymes in FEIAs?
• How does the intensity of fluorescence relate to the concentration of the target molecule in FEIAs?
• What are some common applications of FEIAs?

Related terms

ELISA:

Enzyme-linked immunosorbent assay, a technique that uses enzyme-linked antibodies for detecting antigens

EIA:

Enzyme immunoassay, a method that uses enzymes as labels for antibody or antigen detection

Immunofluorescence:

A technique that uses fluorescent-labeled antibodies to detect specific proteins or antigens in biological samples