Differential interference contrast (DIC) microscopes

Definition

Differential interference contrast (DIC) microscopes use polarized light to enhance contrast in unstained, transparent samples. They are particularly useful for observing live cells and detailed structures within them.

5 Must Know Facts For Your Next Test

1. DIC microscopy utilizes polarized light and Nomarski prisms to produce high-contrast images of transparent specimens.
2. This technique enhances the edges and fine details of samples without the need for staining.
3. DIC is advantageous for observing living cells since it does not require dyes that might be toxic.
4. It creates a pseudo-3D effect that helps in visualizing cellular structures in greater detail.
5. The primary components of a DIC microscope include polarizers, analyzers, and differential interference prisms.

Review Questions

• How does a DIC microscope enhance contrast in unstained samples?
• What are the primary components required for a DIC microscope?
• Why is DIC microscopy especially useful for studying live cells?

Related terms

Polarized Light Microscopy: A technique that uses polarized light to examine birefringent materials.

Phase Contrast Microscopy: A method that converts phase shifts in light passing through a transparent specimen into variations in intensity.

Fluorescence Microscopy:

A type of microscopy that uses fluorescence instead of reflection and absorption to study properties of organic or inorganic substances.