Diaphragm

Definition

The diaphragm in a microscope is a component that controls the amount of light reaching the specimen. Adjusting the diaphragm enhances contrast and resolution of the image.

5 Must Know Facts For Your Next Test

1. The diaphragm can be found below the stage of the microscope.
2. It typically comes in two forms: iris and disc diaphragms.
3. Proper adjustment of the diaphragm is crucial for obtaining clear images at different magnifications.
4. An iris diaphragm consists of overlapping plates that create an adjustable aperture.
5. Using a diaphragm correctly helps improve both contrast and depth of field in microscopic images.

Review Questions

• Where is the diaphragm located on a microscope?
• What are the two common types of diaphragms found in microscopes?
• How does adjusting the diaphragm affect microscopic imaging?

Related terms

Stage: The flat platform where slides are placed for observation under a microscope.

Objective Lens: The lens closest to the specimen, responsible for primary magnification.

Condenser: A component that focuses light onto the specimen, often working in conjunction with the diaphragm.