Darkfield microscope

Definition

A darkfield microscope is a type of light microscope that uses scattered light to illuminate specimens, making them appear bright against a dark background. It is particularly useful for examining live, unstained samples and small structures.

5 Must Know Facts For Your Next Test

1. Darkfield microscopes enhance contrast in unstained samples by using a special condenser to block direct light.
2. They are ideal for observing transparent or colorless specimens like bacteria, spirochetes, and protozoa.
3. The technique helps to view motility and morphology of microorganisms without staining.
4. Darkfield microscopy can reveal fine details not visible with standard brightfield microscopy.
5. It is commonly used in microbiology for rapid detection and identification of pathogens.

Review Questions

• How does a darkfield microscope create contrast in unstained specimens?
• What types of samples are best observed using a darkfield microscope?
• Why is darkfield microscopy advantageous for studying microorganisms?

Related terms

Brightfield Microscope: A microscope that illuminates the specimen directly with white light, making it appear darker against a bright background.

Phase Contrast Microscope: A type of microscope that enhances contrast in transparent specimens by converting phase shifts into changes in amplitude.

Fluorescence Microscope: A microscope that uses high-intensity illumination to excite fluorescent molecules within the specimen, causing them to emit light at different wavelengths.