๐Ÿ”ฌgeneral biology i review

key term - Northern blotting

Definition

Northern blotting is a laboratory technique used to detect specific RNA sequences in a sample. It involves the separation of RNA by gel electrophoresis, transfer to a membrane, and hybridization with a labeled probe.

5 Must Know Facts For Your Next Test

  1. Northern blotting specifically detects RNA molecules.
  2. The process involves gel electrophoresis, membrane transfer, and hybridization with a probe.
  3. It is commonly used to study gene expression levels.
  4. Radioactive or non-radioactive probes can be used for detection.
  5. Northern blotting can provide information about the size and abundance of mRNA transcripts.

Review Questions

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