FACT

Definition

A FACT (Facilitates Chromatin Transcription) complex is a protein complex that reorganizes nucleosomes to make DNA more accessible for transcription. It plays a critical role in maintaining chromatin structure during the transcription process.

5 Must Know Facts For Your Next Test

1. The FACT complex consists of two subunits: SPT16 and SSRP1.
2. FACT can both dismantle and reassemble nucleosomes, aiding RNA polymerase II progression.
3. FACT is essential for the transcription elongation phase in eukaryotic cells.
4. It helps in histone displacement and repositioning to allow RNA polymerase II access to DNA.
5. Mutations or malfunctions in FACT can lead to disruptions in gene expression and are linked to various diseases.

Review Questions

• What are the two subunits that make up the FACT complex?
• How does the FACT complex facilitate transcription elongation?
• Why is the function of FACT crucial for proper gene expression?

Related terms

RNA Polymerase II: An enzyme responsible for transcribing most of the genes in eukaryotic cells.

Nucleosome: The basic structural unit of chromatin, consisting of a segment of DNA wound around histone proteins.

Chromatin: A complex of DNA and protein found in eukaryotic cells that packages DNA into a more compact, dense shape.