Advanced Chemical Engineering Science
Error-prone PCR is a variation of the polymerase chain reaction (PCR) that intentionally introduces mutations into the DNA sequence being amplified. This technique is primarily used in synthetic biology and metabolic engineering to create diversity in genetic material, enabling researchers to study protein function or develop novel enzymes. By increasing the mutation rate during amplification, it allows for the exploration of new biological functions and pathways that can be harnessed for various applications.
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