7.1 Passive diffusion and facilitated transport

6 min readaugust 1, 2024

and facilitated transport are crucial mechanisms for moving molecules across cell membranes. These processes rely on concentration gradients, allowing substances to flow from high to low concentrations without energy input.

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Top images from around the web for Passive Diffusion: Principles and Role
Top images from around the web for Passive Diffusion: Principles and Role
Top images from around the web for Passive Diffusion: Principles and Role

While passive diffusion lets small, nonpolar molecules pass through the membrane directly, facilitated transport uses proteins to help larger or charged molecules cross. Both methods play vital roles in cellular function, , and waste removal.

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Top images from around the web for Passive Diffusion: Principles and Role
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Top images from around the web for Passive Diffusion: Principles and Role

Passive Diffusion: Principles and Role

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Top images from around the web for Passive Diffusion: Principles and Role
Top images from around the web for Passive Diffusion: Principles and Role
Top images from around the web for Passive Diffusion: Principles and Role

Fundamentals of Passive Diffusion

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  • Passive diffusion involves the movement of molecules from a region of high concentration to a region of low concentration, driven by the
Top images from around the web for Passive Diffusion: Principles and Role
Top images from around the web for Passive Diffusion: Principles and Role
  • This process occurs without the expenditure of cellular energy (ATP)
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Top images from around the web for Passive Diffusion: Principles and Role
  • Molecules move down their concentration gradient until equilibrium is reached, at which point the net movement of molecules across the membrane is zero
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Top images from around the web for Passive Diffusion: Principles and Role
  • The rate of passive diffusion is directly proportional to the steepness of the concentration gradient (a larger difference in concentration results in a faster rate of diffusion)
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Passive Diffusion Across Cell Membranes

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  • Passive diffusion occurs through the of the cell membrane, allowing certain molecules to pass through without the aid of
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  • Small, nonpolar molecules (oxygen, carbon dioxide, and certain lipid-soluble molecules like steroids) can easily diffuse through the hydrophobic core of the membrane
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  • The permeability of the cell membrane to different molecules depends on their size, charge, and polarity
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  • Larger, polar, or charged molecules have difficulty passing through the membrane via passive diffusion due to their incompatibility with the hydrophobic interior of the bilayer
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  • Passive diffusion plays a crucial role in the transport of gases across the cell membrane, facilitating (oxygen uptake) and removal of metabolic waste (carbon dioxide release)
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Facilitated Diffusion: Process and Significance

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Mechanism of Facilitated Diffusion

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  • is a type of passive transport that involves the movement of specific molecules across the cell membrane with the help of membrane-bound proteins
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Top images from around the web for Passive Diffusion: Principles and Role
  • undergo conformational changes to bind and transport specific molecules across the membrane
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Top images from around the web for Passive Diffusion: Principles and Role
  • Example: Glucose transporter (GLUT) proteins facilitate the uptake of glucose into cells
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Top images from around the web for Passive Diffusion: Principles and Role
  • form hydrophilic pores that allow the passage of specific ions or small molecules
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Top images from around the web for Passive Diffusion: Principles and Role
  • Example: Potassium ion channels allow the selective movement of K+ ions across the membrane
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  • Facilitated diffusion enables the transport of larger, polar, or charged molecules that cannot easily pass through the phospholipid bilayer
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Significance of Facilitated Diffusion in Biological Systems

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  • Facilitated diffusion is essential for the uptake of nutrients by cells
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Top images from around the web for Passive Diffusion: Principles and Role
  • Glucose and amino acid transporters ensure an adequate supply of these vital molecules for cellular metabolism and growth
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Top images from around the web for Passive Diffusion: Principles and Role
  • Ion channels regulate the concentration of ions (sodium, potassium, calcium) across the membrane, crucial for maintaining the membrane potential and signal transduction
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  • Facilitated diffusion aids in the removal of waste products from cells, such as the transport of urea out of liver cells
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  • The rate of facilitated diffusion is limited by the number of available carrier or channel proteins and their saturation at high substrate concentrations
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Top images from around the web for Passive Diffusion: Principles and Role
  • Saturation occurs when all the binding sites on the proteins are occupied, resulting in a maximum transport rate (Vmax) that cannot be exceeded even with further increases in substrate concentration
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Passive Diffusion vs Facilitated Transport

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Similarities

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Top images from around the web for Passive Diffusion: Principles and Role
  • Both passive diffusion and facilitated diffusion are forms of passive transport that move molecules down their concentration gradient
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Top images from around the web for Passive Diffusion: Principles and Role
  • Neither process requires the expenditure of cellular energy (ATP)
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Top images from around the web for Passive Diffusion: Principles and Role
  • The rate of transport for both mechanisms is influenced by the concentration gradient (a steeper gradient leads to faster transport)
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Top images from around the web for Passive Diffusion: Principles and Role
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Differences

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  • Passive diffusion occurs directly through the phospholipid bilayer, while facilitated diffusion requires the assistance of membrane-bound carrier proteins or channel proteins
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Top images from around the web for Passive Diffusion: Principles and Role
  • Passive diffusion is limited to small, nonpolar molecules, whereas facilitated diffusion can transport larger, polar, or charged molecules
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Top images from around the web for Passive Diffusion: Principles and Role
  • The rate of passive diffusion is directly proportional to the concentration gradient, while the rate of facilitated diffusion is limited by the number and saturation of carrier or channel proteins
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Top images from around the web for Passive Diffusion: Principles and Role
  • Passive diffusion does not exhibit specificity for particular molecules, while facilitated diffusion is highly specific, with proteins transporting only certain molecules or ions
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Factors Influencing Diffusion Rates

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Concentration Gradient

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  • The concentration gradient is a primary factor influencing the rate of both passive diffusion and facilitated transport
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  • A steeper concentration gradient (a larger difference in concentration between the two sides of the membrane) results in a higher rate of molecular movement
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  • As the concentration gradient decreases, the rate of diffusion or transport slows down until equilibrium is reached
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Temperature

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  • Temperature affects the rate of passive diffusion and facilitated transport by influencing the kinetic energy of molecules
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  • Higher temperatures increase the kinetic energy of molecules, causing them to move faster and collide with the membrane more frequently
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Top images from around the web for Passive Diffusion: Principles and Role
  • Consequently, higher temperatures generally increase the rate of diffusion and transport
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Top images from around the web for Passive Diffusion: Principles and Role
  • Lower temperatures decrease kinetic energy and slow down the rate of diffusion and transport
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Membrane Permeability and Composition

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  • Membrane permeability, determined by the composition and structure of the phospholipid bilayer, influences the rate of passive diffusion
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Top images from around the web for Passive Diffusion: Principles and Role
  • More permeable membranes allow for faster diffusion of molecules
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Top images from around the web for Passive Diffusion: Principles and Role
  • The presence of cholesterol in the membrane can reduce permeability by increasing the rigidity of the bilayer and hindering the movement of molecules
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  • The type and proportion of phospholipids in the membrane (such as the ratio of saturated to unsaturated fatty acids) can also affect permeability
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Molecular Properties

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  • The size, charge, and polarity of molecules affect their ability to pass through the cell membrane
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Top images from around the web for Passive Diffusion: Principles and Role
  • Small, nonpolar molecules (carbon dioxide, oxygen) diffuse more readily compared to large, polar, or charged molecules (glucose, amino acids, ions)
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  • Larger molecules experience more resistance when passing through the membrane due to their size and potential interactions with the hydrophobic core of the bilayer
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  • Charged molecules are repelled by the hydrophobic interior of the membrane and require carrier proteins or channel proteins for transport
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Protein Availability and Saturation (Facilitated Diffusion)

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  • In facilitated transport, the number and availability of carrier or channel proteins determine the maximum rate of transport
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  • The presence of more proteins increases the transport capacity, allowing for a higher rate of facilitated diffusion
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  • Saturation of carrier proteins occurs when all the binding sites are occupied by substrate molecules, limiting the rate of transport at high substrate concentrations
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  • Once saturation is reached, further increases in substrate concentration do not result in an increased rate of transport, leading to a plateau in the transport rate
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Inhibitors and Competing Molecules (Facilitated Diffusion)

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  • The presence of inhibitors or competing molecules can reduce the rate of facilitated transport by blocking or occupying the binding sites on carrier or channel proteins
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Top images from around the web for Passive Diffusion: Principles and Role
  • Competitive inhibitors are molecules that closely resemble the substrate and compete for the same binding sites on the proteins, reducing the transport of the desired substrate
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  • Non-competitive inhibitors bind to allosteric sites on the proteins, causing conformational changes that decrease the affinity for the substrate or prevent the transport process altogether
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  • Example: Ouabain, a cardiac glycoside, inhibits the sodium-potassium pump (Na+/K+ ATPase) by binding to the potassium binding site, disrupting the normal transport of sodium and potassium ions across the membrane

Key Terms to Review (18)

Carrier proteins: Carrier proteins are specialized membrane proteins that facilitate the transport of specific substances across a cell membrane. Unlike channels that allow passive movement, carrier proteins undergo conformational changes to bind and transport molecules, ensuring that essential nutrients and ions enter or exit the cell efficiently.
Cellular Respiration: Cellular respiration is a metabolic process that converts biochemical energy from nutrients into adenosine triphosphate (ATP), and releases waste products. This process is essential for producing the energy required by cells to perform various functions, connecting closely with energy transfer, enzyme activity, membrane transport, and thermodynamics in biological systems.
Channel proteins: Channel proteins are specialized membrane proteins that facilitate the transport of ions and molecules across the cell membrane by forming pores or channels. These proteins are essential for various cellular processes, allowing specific substances to pass through the lipid bilayer in a controlled manner, and play a critical role in maintaining homeostasis by regulating the movement of ions and small molecules in and out of cells.
Concentration gradient: A concentration gradient refers to the difference in the concentration of a substance between two areas, typically across a membrane. This gradient is crucial in determining the movement of molecules in biological systems, influencing processes like passive diffusion and facilitated transport, where substances naturally move from areas of higher concentration to areas of lower concentration without the need for energy input.
Facilitated diffusion: Facilitated diffusion is a passive transport process that allows substances to cross membranes with the help of specific transport proteins, without the need for energy input. This mechanism is crucial for the movement of polar and charged molecules, which cannot easily diffuse through the lipid bilayer of cell membranes. It operates along the concentration gradient, ensuring that molecules move from areas of higher concentration to areas of lower concentration, while also being influenced by factors such as membrane potential and electrochemical gradients.
Fluorescence recovery after photobleaching: Fluorescence recovery after photobleaching (FRAP) is a technique used to study the dynamics of molecules within living cells by measuring the recovery of fluorescence in a bleached area over time. This method allows researchers to analyze how quickly and efficiently molecules can move into a region after a portion has been selectively destroyed using intense light. FRAP provides valuable insights into membrane dynamics, molecular interactions, and cellular transport mechanisms.
Membrane proteins: Membrane proteins are essential proteins that are embedded in or associated with biological membranes, playing critical roles in various cellular functions such as signaling, transport, and maintaining the structure of the membrane. These proteins can either span the entire membrane or be anchored to one side, influencing how cells interact with their environment and each other. They are crucial for processes like passive diffusion and facilitated transport, and understanding their structure and behavior is vital for advancements in biophysics and related fields.
Nutrient uptake: Nutrient uptake refers to the process by which cells absorb essential substances, such as minerals, vitamins, and other nutrients, from their surrounding environment. This process is critical for cellular function and growth, as it allows organisms to obtain the building blocks necessary for metabolism, energy production, and overall homeostasis. Different mechanisms, including passive diffusion and facilitated transport, govern how these nutrients are absorbed into cells.
Osmometry: Osmometry is the measurement of the osmotic pressure of solutions, which reflects the concentration of solute particles in a solvent. This technique is crucial for understanding how substances move across membranes via processes like passive diffusion and facilitated transport. It helps in determining how various solutes affect the osmotic balance, which is essential for maintaining cellular homeostasis and function.
Passive diffusion: Passive diffusion is the process by which molecules move across a biological membrane from an area of higher concentration to an area of lower concentration without the use of energy. This movement occurs spontaneously due to the inherent kinetic energy of molecules, and it plays a crucial role in cellular processes, allowing for the exchange of substances essential for life.
Passive vs Active Transport: Passive transport and active transport are two fundamental mechanisms by which substances move across cell membranes. Passive transport occurs without the need for cellular energy, allowing molecules to flow down their concentration gradient, while active transport requires energy input, often in the form of ATP, to move substances against their concentration gradient. Both processes are essential for maintaining cellular homeostasis and facilitating communication between cells.
Phospholipid bilayer: The phospholipid bilayer is a double layer of phospholipids that forms the fundamental structure of cell membranes, providing a semi-permeable barrier that separates the interior of the cell from the external environment. This unique arrangement allows for the selective transport of molecules, crucial for maintaining cellular homeostasis and facilitating processes like passive diffusion and facilitated transport.
Polar molecules: Polar molecules are molecules that have a net dipole moment due to the presence of polar bonds, where the electrons are not shared equally between atoms. This uneven sharing of electrons leads to regions of partial positive and negative charges within the molecule, causing it to exhibit unique properties such as solubility in water and reactivity with other polar substances.
Saturation Kinetics: Saturation kinetics refers to the phenomenon where the rate of a process, such as transport or enzymatic reaction, reaches a maximum velocity as the substrate concentration increases, leading to a plateau in the reaction rate. This occurs because all available binding sites are occupied, and further increases in substrate concentration do not result in a proportional increase in reaction rate. This concept is crucial for understanding how substances move across membranes and how enzymes function in biological systems.
Selective permeability: Selective permeability is the property of cell membranes that allows certain substances to pass through while blocking others. This characteristic is vital for maintaining homeostasis within the cell, as it enables the selective entry and exit of ions, nutrients, and waste products. It is largely dependent on the structure and function of membrane proteins, which facilitate or restrict transport mechanisms across the lipid bilayer.
Small nonpolar molecules: Small nonpolar molecules are chemical species that are generally hydrophobic and do not carry any significant electrical charge, allowing them to easily pass through lipid bilayers. These molecules, such as oxygen, carbon dioxide, and nitrogen, can move across cell membranes without the need for assistance due to their size and lack of polarity. Their properties play a crucial role in cellular processes, particularly in passive diffusion and facilitated transport mechanisms.
Transport maximum: Transport maximum refers to the maximum rate at which a substance can be transported across a cell membrane by specific transport proteins or carriers. This concept is crucial in understanding how substances are moved into and out of cells, especially when dealing with facilitated transport where carrier proteins assist in moving molecules that cannot easily diffuse through the lipid bilayer.
Uniport vs Symport: Uniport and symport are two types of transport mechanisms used by cells to move substances across their membranes. Uniport refers to the process of transporting a single type of molecule or ion across the membrane in one direction, while symport involves the simultaneous transport of two different molecules or ions in the same direction. Both mechanisms are crucial for maintaining cellular homeostasis and facilitating the movement of essential nutrients and ions.
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